(PT) Desenvolvimento de marcadores microssatélites para Brasilianthus carajensis, uma espécie anual e endêmica das Cangas da Serra dos Carajás, Pará, Brasil

Author(s): SILVA, Amanda Reis da; VIANA, Pedro Lage; RIBBI, Rodolfo Jaffé
Summary: Although mining is an essential activity, it causes major changes in the natural landscape, affecting local fauna and flora. The impact on the vegetation of the In Serra dos Carajás caused by mining is evident, and it is worrisome due to the presence of several endemic species in the region. Brasilianthus carajensis is one endemic species with an annual life cycle which is easily found around the mines. Establishing a strategy for the conservation of this species considering the genetic variability of its populations is important to safeguard this species. Microsatellite markers are commonly used to monitor genetic diversity and gene flow. Thus, the aim of this project was to develop microsatellite markers (SSR) for Brasilianthus carajensis, that can be used infuture monitoring studies, assessing the influence of mining activities. A total of 438 samples were collected for DNA extraction, following the 2% CTAB protocol with subsequent purification. Microsatellite markers were identified through Nest Generation Sequencing using the Illumina MiSeq platform, filtered according to QDD program guidelines and tested for amplification. Sequencing identified 30.573 microsatellite markers, of which 31 were selected for amplification. In total, 23 markers were polymorphic and showed good amplification, representing a high number in comparison with other works. The results generated will help future studies assessinggenetic diversity, gene flow, and the influence of mining activities on the populations of this species
Year: 2017
Pages: 40 f.
DOI: https://doi.org/10.29223/PROD.TEC.ITV.DS.2017.8.Silva
Link: http://pergamum.itvds.org:81/vinculos/00000d/00000d01.pdf
Year of publication: 2017
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